Plasmid Preparation:Article Title: Mitohormesis Primes Tumor Invasion and Metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays AEC Peroxidase Substrate Kit Vector Laboratories CatSK-4200; RRID:AB_2336076 Plasmid Maxi Kit QIAGEN Cat#12165 Cell Culture Insert, Transparent PET Membrane, 8 mm pore size Corning Cat#353097; Cat#353093 Hema 3 Manual Staining System and Stat Pack Fisher Scientific Cat#23-123869 Deposited Data Primary breast cancer gene expression data Gene Expression Omnibus GEO: GSE11121, GSE12093, GSE12276, GSE1456, GSE16391, GSE2034, GSE2990, GSE3494, GSE5327, GSE6532, GSE7390, and GSE9195 TCGA Breast Cancer Dataset National Cancer Institute GDC Data Portal TGCA-BRCA, DbGAP Study Accession #phs000178 Experimental Models: Cell Lines MCF7 ATCC Cat#HTB-22 MDA-MB-231 ATCC Cat#HTB-26 Lenti-X 293T Takara Clontech Cat#632180 MMTV-rtTA/TetO-NeuNT mammary tumor cell lines This Paper N/A Experimental Models: Organisms/Strains Mouse: MMTV-rtTA/TetO-NeuNT: FVB Tg(MMTV-rtTA) 1Lach Tg(TetO-Erbb2)1Lach Moody et al., 2002 Mouse Genome Informatics: 5506798 Mouse: FVBN: FVB/NCrl Charles River Strain Code: 207 Mouse: C57BL/6: C57BL/6NCrl Charles River Strain Code: 027 Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo Model Code: 069 Oligonucleotides rtTA Primer Forward 50-TGCCGCCATTATTACGACAAGC-30 This Paper N/A rtTA Primer Reverse 50-ACCGTACTCGTCAATTCCAAGGG-30 This Paper N/A Neu Primer Forward 50-TTTCCTGCAGCAGCCTACGC-30 This Paper N/A Neu Primer Reverse 50-CGGAACCCACATCAGGCC-30 This Paper N/A siSIRT3 #1 Forward 5‘-GCCCAACGUCACUCACUACTT 30 This Paper N/A siSIRT3 #1 Reverse 50-GUAGUGAGUGACGUUGGGCTT-30 This Paper N/A siSIRT3 #2 Forward 5‘-ACUCCCAUUCUUCUUUCACTT-30 This Paper N/A siSIRT3 #2 Reverse 50- GUGAAAGAAGAAUGGGAGUTT-30 This Paper N/A DsiNC1 Integrated DNA Technologies Cat#51-01-14-03 Recombinant DNA shRNA vector set against SIRT3 in pLV-H1-CMV-Green plasmid Biosettia Cat#SORT-B01, Gene ID:23410; Accession NM_001017524.2 Software and Algorithms KM Plotter Györffy et al., 2010; Lánczky et al., 2016 N/A JetSet Li et al., 2011 N/A GraphPad Prism v6.0c https://www.graphpad.com/ N/A FIJI https://fiji.sc/ N/A FCS Express Flow 6 http://www.denovosoftware.com/ site/downloadresearch.shtml N/A GenePattern Reich et al., 2006 N/A Cell Reports 27, 2292–2303.e1–e6, May 21, 2019 e2 ..
Cell Culture:Article Title: Mitohormesis Primes Tumor Invasion and Metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays AEC Peroxidase Substrate Kit Vector Laboratories CatSK-4200; RRID:AB_2336076 Plasmid Maxi Kit QIAGEN Cat#12165 Cell Culture Insert, Transparent PET Membrane, 8 mm pore size Corning Cat#353097; Cat#353093 Hema 3 Manual Staining System and Stat Pack Fisher Scientific Cat#23-123869 Deposited Data Primary breast cancer gene expression data Gene Expression Omnibus GEO: GSE11121, GSE12093, GSE12276, GSE1456, GSE16391, GSE2034, GSE2990, GSE3494, GSE5327, GSE6532, GSE7390, and GSE9195 TCGA Breast Cancer Dataset National Cancer Institute GDC Data Portal TGCA-BRCA, DbGAP Study Accession #phs000178 Experimental Models: Cell Lines MCF7 ATCC Cat#HTB-22 MDA-MB-231 ATCC Cat#HTB-26 Lenti-X 293T Takara Clontech Cat#632180 MMTV-rtTA/TetO-NeuNT mammary tumor cell lines This Paper N/A Experimental Models: Organisms/Strains Mouse: MMTV-rtTA/TetO-NeuNT: FVB Tg(MMTV-rtTA) 1Lach Tg(TetO-Erbb2)1Lach Moody et al., 2002 Mouse Genome Informatics: 5506798 Mouse: FVBN: FVB/NCrl Charles River Strain Code: 207 Mouse: C57BL/6: C57BL/6NCrl Charles River Strain Code: 027 Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo Model Code: 069 Oligonucleotides rtTA Primer Forward 50-TGCCGCCATTATTACGACAAGC-30 This Paper N/A rtTA Primer Reverse 50-ACCGTACTCGTCAATTCCAAGGG-30 This Paper N/A Neu Primer Forward 50-TTTCCTGCAGCAGCCTACGC-30 This Paper N/A Neu Primer Reverse 50-CGGAACCCACATCAGGCC-30 This Paper N/A siSIRT3 #1 Forward 5‘-GCCCAACGUCACUCACUACTT 30 This Paper N/A siSIRT3 #1 Reverse 50-GUAGUGAGUGACGUUGGGCTT-30 This Paper N/A siSIRT3 #2 Forward 5‘-ACUCCCAUUCUUCUUUCACTT-30 This Paper N/A siSIRT3 #2 Reverse 50- GUGAAAGAAGAAUGGGAGUTT-30 This Paper N/A DsiNC1 Integrated DNA Technologies Cat#51-01-14-03 Recombinant DNA shRNA vector set against SIRT3 in pLV-H1-CMV-Green plasmid Biosettia Cat#SORT-B01, Gene ID:23410; Accession NM_001017524.2 Software and Algorithms KM Plotter Györffy et al., 2010; Lánczky et al., 2016 N/A JetSet Li et al., 2011 N/A GraphPad Prism v6.0c https://www.graphpad.com/ N/A FIJI https://fiji.sc/ N/A FCS Express Flow 6 http://www.denovosoftware.com/ site/downloadresearch.shtml N/A GenePattern Reich et al., 2006 N/A Cell Reports 27, 2292–2303.e1–e6, May 21, 2019 e2 ..
Positron Emission Tomography:Article Title: Mitohormesis Primes Tumor Invasion and Metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays AEC Peroxidase Substrate Kit Vector Laboratories CatSK-4200; RRID:AB_2336076 Plasmid Maxi Kit QIAGEN Cat#12165 Cell Culture Insert, Transparent PET Membrane, 8 mm pore size Corning Cat#353097; Cat#353093 Hema 3 Manual Staining System and Stat Pack Fisher Scientific Cat#23-123869 Deposited Data Primary breast cancer gene expression data Gene Expression Omnibus GEO: GSE11121, GSE12093, GSE12276, GSE1456, GSE16391, GSE2034, GSE2990, GSE3494, GSE5327, GSE6532, GSE7390, and GSE9195 TCGA Breast Cancer Dataset National Cancer Institute GDC Data Portal TGCA-BRCA, DbGAP Study Accession #phs000178 Experimental Models: Cell Lines MCF7 ATCC Cat#HTB-22 MDA-MB-231 ATCC Cat#HTB-26 Lenti-X 293T Takara Clontech Cat#632180 MMTV-rtTA/TetO-NeuNT mammary tumor cell lines This Paper N/A Experimental Models: Organisms/Strains Mouse: MMTV-rtTA/TetO-NeuNT: FVB Tg(MMTV-rtTA) 1Lach Tg(TetO-Erbb2)1Lach Moody et al., 2002 Mouse Genome Informatics: 5506798 Mouse: FVBN: FVB/NCrl Charles River Strain Code: 207 Mouse: C57BL/6: C57BL/6NCrl Charles River Strain Code: 027 Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo Model Code: 069 Oligonucleotides rtTA Primer Forward 50-TGCCGCCATTATTACGACAAGC-30 This Paper N/A rtTA Primer Reverse 50-ACCGTACTCGTCAATTCCAAGGG-30 This Paper N/A Neu Primer Forward 50-TTTCCTGCAGCAGCCTACGC-30 This Paper N/A Neu Primer Reverse 50-CGGAACCCACATCAGGCC-30 This Paper N/A siSIRT3 #1 Forward 5‘-GCCCAACGUCACUCACUACTT 30 This Paper N/A siSIRT3 #1 Reverse 50-GUAGUGAGUGACGUUGGGCTT-30 This Paper N/A siSIRT3 #2 Forward 5‘-ACUCCCAUUCUUCUUUCACTT-30 This Paper N/A siSIRT3 #2 Reverse 50- GUGAAAGAAGAAUGGGAGUTT-30 This Paper N/A DsiNC1 Integrated DNA Technologies Cat#51-01-14-03 Recombinant DNA shRNA vector set against SIRT3 in pLV-H1-CMV-Green plasmid Biosettia Cat#SORT-B01, Gene ID:23410; Accession NM_001017524.2 Software and Algorithms KM Plotter Györffy et al., 2010; Lánczky et al., 2016 N/A JetSet Li et al., 2011 N/A GraphPad Prism v6.0c https://www.graphpad.com/ N/A FIJI https://fiji.sc/ N/A FCS Express Flow 6 http://www.denovosoftware.com/ site/downloadresearch.shtml N/A GenePattern Reich et al., 2006 N/A Cell Reports 27, 2292–2303.e1–e6, May 21, 2019 e2 ..
Membrane:Article Title: Mitohormesis Primes Tumor Invasion and Metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays AEC Peroxidase Substrate Kit Vector Laboratories CatSK-4200; RRID:AB_2336076 Plasmid Maxi Kit QIAGEN Cat#12165 Cell Culture Insert, Transparent PET Membrane, 8 mm pore size Corning Cat#353097; Cat#353093 Hema 3 Manual Staining System and Stat Pack Fisher Scientific Cat#23-123869 Deposited Data Primary breast cancer gene expression data Gene Expression Omnibus GEO: GSE11121, GSE12093, GSE12276, GSE1456, GSE16391, GSE2034, GSE2990, GSE3494, GSE5327, GSE6532, GSE7390, and GSE9195 TCGA Breast Cancer Dataset National Cancer Institute GDC Data Portal TGCA-BRCA, DbGAP Study Accession #phs000178 Experimental Models: Cell Lines MCF7 ATCC Cat#HTB-22 MDA-MB-231 ATCC Cat#HTB-26 Lenti-X 293T Takara Clontech Cat#632180 MMTV-rtTA/TetO-NeuNT mammary tumor cell lines This Paper N/A Experimental Models: Organisms/Strains Mouse: MMTV-rtTA/TetO-NeuNT: FVB Tg(MMTV-rtTA) 1Lach Tg(TetO-Erbb2)1Lach Moody et al., 2002 Mouse Genome Informatics: 5506798 Mouse: FVBN: FVB/NCrl Charles River Strain Code: 207 Mouse: C57BL/6: C57BL/6NCrl Charles River Strain Code: 027 Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo Model Code: 069 Oligonucleotides rtTA Primer Forward 50-TGCCGCCATTATTACGACAAGC-30 This Paper N/A rtTA Primer Reverse 50-ACCGTACTCGTCAATTCCAAGGG-30 This Paper N/A Neu Primer Forward 50-TTTCCTGCAGCAGCCTACGC-30 This Paper N/A Neu Primer Reverse 50-CGGAACCCACATCAGGCC-30 This Paper N/A siSIRT3 #1 Forward 5‘-GCCCAACGUCACUCACUACTT 30 This Paper N/A siSIRT3 #1 Reverse 50-GUAGUGAGUGACGUUGGGCTT-30 This Paper N/A siSIRT3 #2 Forward 5‘-ACUCCCAUUCUUCUUUCACTT-30 This Paper N/A siSIRT3 #2 Reverse 50- GUGAAAGAAGAAUGGGAGUTT-30 This Paper N/A DsiNC1 Integrated DNA Technologies Cat#51-01-14-03 Recombinant DNA shRNA vector set against SIRT3 in pLV-H1-CMV-Green plasmid Biosettia Cat#SORT-B01, Gene ID:23410; Accession NM_001017524.2 Software and Algorithms KM Plotter Györffy et al., 2010; Lánczky et al., 2016 N/A JetSet Li et al., 2011 N/A GraphPad Prism v6.0c https://www.graphpad.com/ N/A FIJI https://fiji.sc/ N/A FCS Express Flow 6 http://www.denovosoftware.com/ site/downloadresearch.shtml N/A GenePattern Reich et al., 2006 N/A Cell Reports 27, 2292–2303.e1–e6, May 21, 2019 e2 ..
Pore Size:Article Title: Mitohormesis Primes Tumor Invasion and Metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays AEC Peroxidase Substrate Kit Vector Laboratories CatSK-4200; RRID:AB_2336076 Plasmid Maxi Kit QIAGEN Cat#12165 Cell Culture Insert, Transparent PET Membrane, 8 mm pore size Corning Cat#353097; Cat#353093 Hema 3 Manual Staining System and Stat Pack Fisher Scientific Cat#23-123869 Deposited Data Primary breast cancer gene expression data Gene Expression Omnibus GEO: GSE11121, GSE12093, GSE12276, GSE1456, GSE16391, GSE2034, GSE2990, GSE3494, GSE5327, GSE6532, GSE7390, and GSE9195 TCGA Breast Cancer Dataset National Cancer Institute GDC Data Portal TGCA-BRCA, DbGAP Study Accession #phs000178 Experimental Models: Cell Lines MCF7 ATCC Cat#HTB-22 MDA-MB-231 ATCC Cat#HTB-26 Lenti-X 293T Takara Clontech Cat#632180 MMTV-rtTA/TetO-NeuNT mammary tumor cell lines This Paper N/A Experimental Models: Organisms/Strains Mouse: MMTV-rtTA/TetO-NeuNT: FVB Tg(MMTV-rtTA) 1Lach Tg(TetO-Erbb2)1Lach Moody et al., 2002 Mouse Genome Informatics: 5506798 Mouse: FVBN: FVB/NCrl Charles River Strain Code: 207 Mouse: C57BL/6: C57BL/6NCrl Charles River Strain Code: 027 Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo Model Code: 069 Oligonucleotides rtTA Primer Forward 50-TGCCGCCATTATTACGACAAGC-30 This Paper N/A rtTA Primer Reverse 50-ACCGTACTCGTCAATTCCAAGGG-30 This Paper N/A Neu Primer Forward 50-TTTCCTGCAGCAGCCTACGC-30 This Paper N/A Neu Primer Reverse 50-CGGAACCCACATCAGGCC-30 This Paper N/A siSIRT3 #1 Forward 5‘-GCCCAACGUCACUCACUACTT 30 This Paper N/A siSIRT3 #1 Reverse 50-GUAGUGAGUGACGUUGGGCTT-30 This Paper N/A siSIRT3 #2 Forward 5‘-ACUCCCAUUCUUCUUUCACTT-30 This Paper N/A siSIRT3 #2 Reverse 50- GUGAAAGAAGAAUGGGAGUTT-30 This Paper N/A DsiNC1 Integrated DNA Technologies Cat#51-01-14-03 Recombinant DNA shRNA vector set against SIRT3 in pLV-H1-CMV-Green plasmid Biosettia Cat#SORT-B01, Gene ID:23410; Accession NM_001017524.2 Software and Algorithms KM Plotter Györffy et al., 2010; Lánczky et al., 2016 N/A JetSet Li et al., 2011 N/A GraphPad Prism v6.0c https://www.graphpad.com/ N/A FIJI https://fiji.sc/ N/A FCS Express Flow 6 http://www.denovosoftware.com/ site/downloadresearch.shtml N/A GenePattern Reich et al., 2006 N/A Cell Reports 27, 2292–2303.e1–e6, May 21, 2019 e2 ..
Staining:Article Title: Mitohormesis Primes Tumor Invasion and Metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays AEC Peroxidase Substrate Kit Vector Laboratories CatSK-4200; RRID:AB_2336076 Plasmid Maxi Kit QIAGEN Cat#12165 Cell Culture Insert, Transparent PET Membrane, 8 mm pore size Corning Cat#353097; Cat#353093 Hema 3 Manual Staining System and Stat Pack Fisher Scientific Cat#23-123869 Deposited Data Primary breast cancer gene expression data Gene Expression Omnibus GEO: GSE11121, GSE12093, GSE12276, GSE1456, GSE16391, GSE2034, GSE2990, GSE3494, GSE5327, GSE6532, GSE7390, and GSE9195 TCGA Breast Cancer Dataset National Cancer Institute GDC Data Portal TGCA-BRCA, DbGAP Study Accession #phs000178 Experimental Models: Cell Lines MCF7 ATCC Cat#HTB-22 MDA-MB-231 ATCC Cat#HTB-26 Lenti-X 293T Takara Clontech Cat#632180 MMTV-rtTA/TetO-NeuNT mammary tumor cell lines This Paper N/A Experimental Models: Organisms/Strains Mouse: MMTV-rtTA/TetO-NeuNT: FVB Tg(MMTV-rtTA) 1Lach Tg(TetO-Erbb2)1Lach Moody et al., 2002 Mouse Genome Informatics: 5506798 Mouse: FVBN: FVB/NCrl Charles River Strain Code: 207 Mouse: C57BL/6: C57BL/6NCrl Charles River Strain Code: 027 Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo Model Code: 069 Oligonucleotides rtTA Primer Forward 50-TGCCGCCATTATTACGACAAGC-30 This Paper N/A rtTA Primer Reverse 50-ACCGTACTCGTCAATTCCAAGGG-30 This Paper N/A Neu Primer Forward 50-TTTCCTGCAGCAGCCTACGC-30 This Paper N/A Neu Primer Reverse 50-CGGAACCCACATCAGGCC-30 This Paper N/A siSIRT3 #1 Forward 5‘-GCCCAACGUCACUCACUACTT 30 This Paper N/A siSIRT3 #1 Reverse 50-GUAGUGAGUGACGUUGGGCTT-30 This Paper N/A siSIRT3 #2 Forward 5‘-ACUCCCAUUCUUCUUUCACTT-30 This Paper N/A siSIRT3 #2 Reverse 50- GUGAAAGAAGAAUGGGAGUTT-30 This Paper N/A DsiNC1 Integrated DNA Technologies Cat#51-01-14-03 Recombinant DNA shRNA vector set against SIRT3 in pLV-H1-CMV-Green plasmid Biosettia Cat#SORT-B01, Gene ID:23410; Accession NM_001017524.2 Software and Algorithms KM Plotter Györffy et al., 2010; Lánczky et al., 2016 N/A JetSet Li et al., 2011 N/A GraphPad Prism v6.0c https://www.graphpad.com/ N/A FIJI https://fiji.sc/ N/A FCS Express Flow 6 http://www.denovosoftware.com/ site/downloadresearch.shtml N/A GenePattern Reich et al., 2006 N/A Cell Reports 27, 2292–2303.e1–e6, May 21, 2019 e2 ..
Gene Expression:Article Title: Mitohormesis Primes Tumor Invasion and Metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays AEC Peroxidase Substrate Kit Vector Laboratories CatSK-4200; RRID:AB_2336076 Plasmid Maxi Kit QIAGEN Cat#12165 Cell Culture Insert, Transparent PET Membrane, 8 mm pore size Corning Cat#353097; Cat#353093 Hema 3 Manual Staining System and Stat Pack Fisher Scientific Cat#23-123869 Deposited Data Primary breast cancer gene expression data Gene Expression Omnibus GEO: GSE11121, GSE12093, GSE12276, GSE1456, GSE16391, GSE2034, GSE2990, GSE3494, GSE5327, GSE6532, GSE7390, and GSE9195 TCGA Breast Cancer Dataset National Cancer Institute GDC Data Portal TGCA-BRCA, DbGAP Study Accession #phs000178 Experimental Models: Cell Lines MCF7 ATCC Cat#HTB-22 MDA-MB-231 ATCC Cat#HTB-26 Lenti-X 293T Takara Clontech Cat#632180 MMTV-rtTA/TetO-NeuNT mammary tumor cell lines This Paper N/A Experimental Models: Organisms/Strains Mouse: MMTV-rtTA/TetO-NeuNT: FVB Tg(MMTV-rtTA) 1Lach Tg(TetO-Erbb2)1Lach Moody et al., 2002 Mouse Genome Informatics: 5506798 Mouse: FVBN: FVB/NCrl Charles River Strain Code: 207 Mouse: C57BL/6: C57BL/6NCrl Charles River Strain Code: 027 Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo Model Code: 069 Oligonucleotides rtTA Primer Forward 50-TGCCGCCATTATTACGACAAGC-30 This Paper N/A rtTA Primer Reverse 50-ACCGTACTCGTCAATTCCAAGGG-30 This Paper N/A Neu Primer Forward 50-TTTCCTGCAGCAGCCTACGC-30 This Paper N/A Neu Primer Reverse 50-CGGAACCCACATCAGGCC-30 This Paper N/A siSIRT3 #1 Forward 5‘-GCCCAACGUCACUCACUACTT 30 This Paper N/A siSIRT3 #1 Reverse 50-GUAGUGAGUGACGUUGGGCTT-30 This Paper N/A siSIRT3 #2 Forward 5‘-ACUCCCAUUCUUCUUUCACTT-30 This Paper N/A siSIRT3 #2 Reverse 50- GUGAAAGAAGAAUGGGAGUTT-30 This Paper N/A DsiNC1 Integrated DNA Technologies Cat#51-01-14-03 Recombinant DNA shRNA vector set against SIRT3 in pLV-H1-CMV-Green plasmid Biosettia Cat#SORT-B01, Gene ID:23410; Accession NM_001017524.2 Software and Algorithms KM Plotter Györffy et al., 2010; Lánczky et al., 2016 N/A JetSet Li et al., 2011 N/A GraphPad Prism v6.0c https://www.graphpad.com/ N/A FIJI https://fiji.sc/ N/A FCS Express Flow 6 http://www.denovosoftware.com/ site/downloadresearch.shtml N/A GenePattern Reich et al., 2006 N/A Cell Reports 27, 2292–2303.e1–e6, May 21, 2019 e2 ..
Multiple Displacement Amplification:Article Title: Mitohormesis Primes Tumor Invasion and Metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays AEC Peroxidase Substrate Kit Vector Laboratories CatSK-4200; RRID:AB_2336076 Plasmid Maxi Kit QIAGEN Cat#12165 Cell Culture Insert, Transparent PET Membrane, 8 mm pore size Corning Cat#353097; Cat#353093 Hema 3 Manual Staining System and Stat Pack Fisher Scientific Cat#23-123869 Deposited Data Primary breast cancer gene expression data Gene Expression Omnibus GEO: GSE11121, GSE12093, GSE12276, GSE1456, GSE16391, GSE2034, GSE2990, GSE3494, GSE5327, GSE6532, GSE7390, and GSE9195 TCGA Breast Cancer Dataset National Cancer Institute GDC Data Portal TGCA-BRCA, DbGAP Study Accession #phs000178 Experimental Models: Cell Lines MCF7 ATCC Cat#HTB-22 MDA-MB-231 ATCC Cat#HTB-26 Lenti-X 293T Takara Clontech Cat#632180 MMTV-rtTA/TetO-NeuNT mammary tumor cell lines This Paper N/A Experimental Models: Organisms/Strains Mouse: MMTV-rtTA/TetO-NeuNT: FVB Tg(MMTV-rtTA) 1Lach Tg(TetO-Erbb2)1Lach Moody et al., 2002 Mouse Genome Informatics: 5506798 Mouse: FVBN: FVB/NCrl Charles River Strain Code: 207 Mouse: C57BL/6: C57BL/6NCrl Charles River Strain Code: 027 Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo Model Code: 069 Oligonucleotides rtTA Primer Forward 50-TGCCGCCATTATTACGACAAGC-30 This Paper N/A rtTA Primer Reverse 50-ACCGTACTCGTCAATTCCAAGGG-30 This Paper N/A Neu Primer Forward 50-TTTCCTGCAGCAGCCTACGC-30 This Paper N/A Neu Primer Reverse 50-CGGAACCCACATCAGGCC-30 This Paper N/A siSIRT3 #1 Forward 5‘-GCCCAACGUCACUCACUACTT 30 This Paper N/A siSIRT3 #1 Reverse 50-GUAGUGAGUGACGUUGGGCTT-30 This Paper N/A siSIRT3 #2 Forward 5‘-ACUCCCAUUCUUCUUUCACTT-30 This Paper N/A siSIRT3 #2 Reverse 50- GUGAAAGAAGAAUGGGAGUTT-30 This Paper N/A DsiNC1 Integrated DNA Technologies Cat#51-01-14-03 Recombinant DNA shRNA vector set against SIRT3 in pLV-H1-CMV-Green plasmid Biosettia Cat#SORT-B01, Gene ID:23410; Accession NM_001017524.2 Software and Algorithms KM Plotter Györffy et al., 2010; Lánczky et al., 2016 N/A JetSet Li et al., 2011 N/A GraphPad Prism v6.0c https://www.graphpad.com/ N/A FIJI https://fiji.sc/ N/A FCS Express Flow 6 http://www.denovosoftware.com/ site/downloadresearch.shtml N/A GenePattern Reich et al., 2006 N/A Cell Reports 27, 2292–2303.e1–e6, May 21, 2019 e2 ..
Recombinant:Article Title: Mitohormesis Primes Tumor Invasion and Metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays AEC Peroxidase Substrate Kit Vector Laboratories CatSK-4200; RRID:AB_2336076 Plasmid Maxi Kit QIAGEN Cat#12165 Cell Culture Insert, Transparent PET Membrane, 8 mm pore size Corning Cat#353097; Cat#353093 Hema 3 Manual Staining System and Stat Pack Fisher Scientific Cat#23-123869 Deposited Data Primary breast cancer gene expression data Gene Expression Omnibus GEO: GSE11121, GSE12093, GSE12276, GSE1456, GSE16391, GSE2034, GSE2990, GSE3494, GSE5327, GSE6532, GSE7390, and GSE9195 TCGA Breast Cancer Dataset National Cancer Institute GDC Data Portal TGCA-BRCA, DbGAP Study Accession #phs000178 Experimental Models: Cell Lines MCF7 ATCC Cat#HTB-22 MDA-MB-231 ATCC Cat#HTB-26 Lenti-X 293T Takara Clontech Cat#632180 MMTV-rtTA/TetO-NeuNT mammary tumor cell lines This Paper N/A Experimental Models: Organisms/Strains Mouse: MMTV-rtTA/TetO-NeuNT: FVB Tg(MMTV-rtTA) 1Lach Tg(TetO-Erbb2)1Lach Moody et al., 2002 Mouse Genome Informatics: 5506798 Mouse: FVBN: FVB/NCrl Charles River Strain Code: 207 Mouse: C57BL/6: C57BL/6NCrl Charles River Strain Code: 027 Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo Model Code: 069 Oligonucleotides rtTA Primer Forward 50-TGCCGCCATTATTACGACAAGC-30 This Paper N/A rtTA Primer Reverse 50-ACCGTACTCGTCAATTCCAAGGG-30 This Paper N/A Neu Primer Forward 50-TTTCCTGCAGCAGCCTACGC-30 This Paper N/A Neu Primer Reverse 50-CGGAACCCACATCAGGCC-30 This Paper N/A siSIRT3 #1 Forward 5‘-GCCCAACGUCACUCACUACTT 30 This Paper N/A siSIRT3 #1 Reverse 50-GUAGUGAGUGACGUUGGGCTT-30 This Paper N/A siSIRT3 #2 Forward 5‘-ACUCCCAUUCUUCUUUCACTT-30 This Paper N/A siSIRT3 #2 Reverse 50- GUGAAAGAAGAAUGGGAGUTT-30 This Paper N/A DsiNC1 Integrated DNA Technologies Cat#51-01-14-03 Recombinant DNA shRNA vector set against SIRT3 in pLV-H1-CMV-Green plasmid Biosettia Cat#SORT-B01, Gene ID:23410; Accession NM_001017524.2 Software and Algorithms KM Plotter Györffy et al., 2010; Lánczky et al., 2016 N/A JetSet Li et al., 2011 N/A GraphPad Prism v6.0c https://www.graphpad.com/ N/A FIJI https://fiji.sc/ N/A FCS Express Flow 6 http://www.denovosoftware.com/ site/downloadresearch.shtml N/A GenePattern Reich et al., 2006 N/A Cell Reports 27, 2292–2303.e1–e6, May 21, 2019 e2 ..
shRNA:Article Title: Mitohormesis Primes Tumor Invasion and Metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays AEC Peroxidase Substrate Kit Vector Laboratories CatSK-4200; RRID:AB_2336076 Plasmid Maxi Kit QIAGEN Cat#12165 Cell Culture Insert, Transparent PET Membrane, 8 mm pore size Corning Cat#353097; Cat#353093 Hema 3 Manual Staining System and Stat Pack Fisher Scientific Cat#23-123869 Deposited Data Primary breast cancer gene expression data Gene Expression Omnibus GEO: GSE11121, GSE12093, GSE12276, GSE1456, GSE16391, GSE2034, GSE2990, GSE3494, GSE5327, GSE6532, GSE7390, and GSE9195 TCGA Breast Cancer Dataset National Cancer Institute GDC Data Portal TGCA-BRCA, DbGAP Study Accession #phs000178 Experimental Models: Cell Lines MCF7 ATCC Cat#HTB-22 MDA-MB-231 ATCC Cat#HTB-26 Lenti-X 293T Takara Clontech Cat#632180 MMTV-rtTA/TetO-NeuNT mammary tumor cell lines This Paper N/A Experimental Models: Organisms/Strains Mouse: MMTV-rtTA/TetO-NeuNT: FVB Tg(MMTV-rtTA) 1Lach Tg(TetO-Erbb2)1Lach Moody et al., 2002 Mouse Genome Informatics: 5506798 Mouse: FVBN: FVB/NCrl Charles River Strain Code: 207 Mouse: C57BL/6: C57BL/6NCrl Charles River Strain Code: 027 Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo Model Code: 069 Oligonucleotides rtTA Primer Forward 50-TGCCGCCATTATTACGACAAGC-30 This Paper N/A rtTA Primer Reverse 50-ACCGTACTCGTCAATTCCAAGGG-30 This Paper N/A Neu Primer Forward 50-TTTCCTGCAGCAGCCTACGC-30 This Paper N/A Neu Primer Reverse 50-CGGAACCCACATCAGGCC-30 This Paper N/A siSIRT3 #1 Forward 5‘-GCCCAACGUCACUCACUACTT 30 This Paper N/A siSIRT3 #1 Reverse 50-GUAGUGAGUGACGUUGGGCTT-30 This Paper N/A siSIRT3 #2 Forward 5‘-ACUCCCAUUCUUCUUUCACTT-30 This Paper N/A siSIRT3 #2 Reverse 50- GUGAAAGAAGAAUGGGAGUTT-30 This Paper N/A DsiNC1 Integrated DNA Technologies Cat#51-01-14-03 Recombinant DNA shRNA vector set against SIRT3 in pLV-H1-CMV-Green plasmid Biosettia Cat#SORT-B01, Gene ID:23410; Accession NM_001017524.2 Software and Algorithms KM Plotter Györffy et al., 2010; Lánczky et al., 2016 N/A JetSet Li et al., 2011 N/A GraphPad Prism v6.0c https://www.graphpad.com/ N/A FIJI https://fiji.sc/ N/A FCS Express Flow 6 http://www.denovosoftware.com/ site/downloadresearch.shtml N/A GenePattern Reich et al., 2006 N/A Cell Reports 27, 2292–2303.e1–e6, May 21, 2019 e2 ..
Software:Article Title: Mitohormesis Primes Tumor Invasion and Metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays AEC Peroxidase Substrate Kit Vector Laboratories CatSK-4200; RRID:AB_2336076 Plasmid Maxi Kit QIAGEN Cat#12165 Cell Culture Insert, Transparent PET Membrane, 8 mm pore size Corning Cat#353097; Cat#353093 Hema 3 Manual Staining System and Stat Pack Fisher Scientific Cat#23-123869 Deposited Data Primary breast cancer gene expression data Gene Expression Omnibus GEO: GSE11121, GSE12093, GSE12276, GSE1456, GSE16391, GSE2034, GSE2990, GSE3494, GSE5327, GSE6532, GSE7390, and GSE9195 TCGA Breast Cancer Dataset National Cancer Institute GDC Data Portal TGCA-BRCA, DbGAP Study Accession #phs000178 Experimental Models: Cell Lines MCF7 ATCC Cat#HTB-22 MDA-MB-231 ATCC Cat#HTB-26 Lenti-X 293T Takara Clontech Cat#632180 MMTV-rtTA/TetO-NeuNT mammary tumor cell lines This Paper N/A Experimental Models: Organisms/Strains Mouse: MMTV-rtTA/TetO-NeuNT: FVB Tg(MMTV-rtTA) 1Lach Tg(TetO-Erbb2)1Lach Moody et al., 2002 Mouse Genome Informatics: 5506798 Mouse: FVBN: FVB/NCrl Charles River Strain Code: 207 Mouse: C57BL/6: C57BL/6NCrl Charles River Strain Code: 027 Mouse: Nude: Hsd:Athymic Nude-Foxn1nu Envigo Model Code: 069 Oligonucleotides rtTA Primer Forward 50-TGCCGCCATTATTACGACAAGC-30 This Paper N/A rtTA Primer Reverse 50-ACCGTACTCGTCAATTCCAAGGG-30 This Paper N/A Neu Primer Forward 50-TTTCCTGCAGCAGCCTACGC-30 This Paper N/A Neu Primer Reverse 50-CGGAACCCACATCAGGCC-30 This Paper N/A siSIRT3 #1 Forward 5‘-GCCCAACGUCACUCACUACTT 30 This Paper N/A siSIRT3 #1 Reverse 50-GUAGUGAGUGACGUUGGGCTT-30 This Paper N/A siSIRT3 #2 Forward 5‘-ACUCCCAUUCUUCUUUCACTT-30 This Paper N/A siSIRT3 #2 Reverse 50- GUGAAAGAAGAAUGGGAGUTT-30 This Paper N/A DsiNC1 Integrated DNA Technologies Cat#51-01-14-03 Recombinant DNA shRNA vector set against SIRT3 in pLV-H1-CMV-Green plasmid Biosettia Cat#SORT-B01, Gene ID:23410; Accession NM_001017524.2 Software and Algorithms KM Plotter Györffy et al., 2010; Lánczky et al., 2016 N/A JetSet Li et al., 2011 N/A GraphPad Prism v6.0c https://www.graphpad.com/ N/A FIJI https://fiji.sc/ N/A FCS Express Flow 6 http://www.denovosoftware.com/ site/downloadresearch.shtml N/A GenePattern Reich et al., 2006 N/A Cell Reports 27, 2292–2303.e1–e6, May 21, 2019 e2 ..
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